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Use of a magnetic covalent organic framework material with a large specific surface area as an effective adsorbent for the extraction and determination of six fluoroquinolone antibiotics by HPLC in milk sample
Author(s) -
Guan Shuping,
Wu Hao,
Yang Liu,
Wang Zhuliang,
Wu Jianmin
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202000616
Subject(s) - adsorption , desorption , chemistry , solid phase extraction , detection limit , extraction (chemistry) , chromatography , lomefloxacin , analytical chemistry (journal) , fourier transform infrared spectroscopy , materials science , norfloxacin , chemical engineering , organic chemistry , biochemistry , ciprofloxacin , engineering , antibiotics
A magnetic covalent organic framework material was synthesized with a core‐shell structure using a simple solvothermal method. It was prepared with Fe 3 O 4 as the magnetic core, covalent organic framework as the shell, which synthesized from 1,3,5‐triformylphloroglucinol and p ‐phenylenediamine by Schiff base reaction. Transmission electron microscopy, Fourier transform infrared spectroscopy, powder X‐ray diffraction, vibrating sample magnetometry, and nitrogen adsorption‐desorption were used to characterize magnetic adsorbent. It has showed a large specific surface area (505.6 m 2 /g), which can provide many adsorption sites. Moreover, the saturation magnetization value was 48.4 emu/g enough to be separated by external magnet. Six kinds of fluoroquinolones (enoxacin, fleroxacin, ofloxacin, norfloxacin, pefloxacin, and lomefloxacin) were extracted by magnetic solid phase extraction with the magnetic adsorbent. High‐performance liquid chromatography detects the entire adsorption and desorption process to further evaluate the optimal extraction and desorption conditions. Under the optimal chromatographic conditions, this method showed a low detection limit (0.05 to 0.20 μg/L), good linearity in the range of 0.5 to 200 μg/L, and the enrichment factor reaches 115.5‐127.3. The spiked recovery of the fluoroquinolones in milk sample ranged from 90.4 to 101.2%.