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Identification of lipophilic components in Citri Reticulatae Pericarpium cultivars by supercritical CO 2 fluid extraction with ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry
Author(s) -
Zheng Guodong,
Liu Mengshi,
Chao Yingxin,
Yang Yuhua,
Zhang Dedong,
Tao Yiwen,
Zhang Jianye,
Zeng Caifang,
Wei Minyan
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202000490
Subject(s) - chemistry , chromatography , mass spectrometry , tandem mass spectrometry , terpene , extraction (chemistry) , supercritical fluid extraction , orbitrap , supercritical fluid chromatography , supercritical fluid , triterpene , high performance liquid chromatography , organic chemistry , medicine , alternative medicine , pathology
To systematically identify the lipophilic constituents of Citri Reticulatae Pericarpium from different cultivars, supercritical CO 2 fluid extraction and ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry were integrated for the component analysis of 18 batches of Citri Reticulatae Pericarpium from 12 cultivars for the first time. A total of 57 components from the supercritical CO 2 fluid extracts were demonstrably or tentatively identified by the obtained parent peaks, fragment peaks, and retention times. In total, two flavonoids, six organic acids, nine coumarins, three aldehydes, seven esters, three terpenes, one limonoid, and five other compounds were detected for the first time; notably, coumarin components have not yet been reported in Citri Reticulatae Pericarpium. Furthermore, the extract constituents differed between cultivars. In particular, organic acids were more abundant in Citrus reticulata “Chachi” than in other cultivars, and pterostilbene was exclusively found in Citrus reticulata “Yichangju”. The results showed that a greater variety of compounds in Citri Reticulatae Pericarpium could be extracted by supercritical CO 2 fluid extraction and detected by ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry. This study provides a more scientific basis for further analysis of the pharmacological activity and quality of Citri Reticulatae Pericarpium components from different cultivars.