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Targeted analysis of six emerging derivatives or metabolites together with 25 common macrolides in milk using Quick, Easy, Cheap, Effective, Rugged and Safe extraction and ultra‐performance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry
Author(s) -
Wang Jia,
Ling Yun,
Zhou Weie,
Li Donghui,
Deng Yamei,
Yang Xusheng,
Zhang Feng
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202000408
Subject(s) - chromatography , chemistry , formic acid , orbitrap , ammonium acetate , mass spectrometry , detection limit , extraction (chemistry) , elution , analyte , electrospray , electrospray ionization , sample preparation , high performance liquid chromatography
An analytical method for the determination of six emerging derivatives or metabolites together with 25 common macrolides antibiotics in milk by ultra‐performance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry was established. The samples were purified with optimized Quick, Easy, Cheap, Effective, Rugged, Safe methods. The amounts of primary‐secondary amine, C18, and sodium acetate adsorbent materials were optimized by response surface method to obtain the best purification effect. The chromatographic separation was carried out using the XBridge‐C18 (2.1 × 100 mm, 3.5 µm, Waters) column with mobile phase of acetonitrile with 0.1% v/v formic acid‐water solutions (containing 10 mmol/L ammonium acetate), separated by gradient elution. The instrument was operated in the detection mode of electrospray positive and negative ions with Full MS/data dependent MS 2 acquisition mode detection, external standard method was used for quantitative analysis. The limits of detection and limits of quantitation of 31 compounds were 0.1–0.5 µg/L and 0.5–2.0 µg/L, respectively. A total of 31 compounds performed a good linearity in the range of 1 to 200 µg/L, and the correlation coefficient was greater than 0.990. The spiked recoveries in milk samples were 81.07–110.1% and the relative standard deviation was less than 5.1%. The method was successful applied to actual sample testing in the market.