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Two‐dimensional liquid chromatography analysis of all‐ trans‐ , 9‐ cis‐ , and 13‐ cis‐ astaxanthin in raw extracts from Phaffia rhodozyma
Author(s) -
Du Xiping,
Wang Chun,
Wu Ling,
Li Zhipeng,
Sadiq Faizan Ahmed,
Jiang Zedong,
Chen Feng,
Ni Hui,
Li Qingbiao
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202000257
Subject(s) - astaxanthin , chromatography , chemistry , cis–trans isomerism , high performance liquid chromatography , column chromatography , carotenoid , stereochemistry , biochemistry
An effective two‐dimensional liquid chromatography method has been established for the analysis of all ‐trans‐ astaxanthin and its geometric isomers from Phaffia rhodozyma employing a C18 column at the first dimension and a C30 column in the second dimension, connected by a 10‐port valve using the photo‐diode array detector. The regression equation of astaxanthin calibration curve was established, and the precision and accuracy values were found to be in the range of 0.32–1.14% and 98.21–106.13%, respectively. By using two‐dimensional liquid chromatography, it was found that day light, ultrasonic treatment, and heat treatment have significant influence on the content of all ‐trans ‐astaxanthin in the extract from P. rhodozyma due to the transformation of all ‐trans ‐astaxanthin to cis‐ astaxanthin. The day light and ultrasonic treatments more likely transform all ‐trans ‐astaxanthin to 9 ‐cis ‐astaxanthin, and the thermal treatment transforms all ‐trans ‐astaxanthin to 13 ‐cis ‐astaxanthin. These results indicate that the two‐dimensional liquid chromatography method can facilitate monitoring astaxanthin isomerization in the raw extract from P. rhodozyma . In addition, the study will provide a general reference for monitoring other medicals and bioactive chemicals with geometric isomers.