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Dispersive solid‐phase extraction of buprenorphine from biological fluids using metal‐organic frameworks and its determination by ultra‐performance liquid chromatography
Author(s) -
Mohammadi Farzaneh,
Shabani Ali Mohammad Haji,
Dadfarnia Shayessteh,
Ansari Mehdi,
Asgharinezhad Ali Akbar
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.202000221
Subject(s) - extraction (chemistry) , chromatography , detection limit , buprenorphine , solid phase extraction , chemistry , high performance liquid chromatography , biochemistry , receptor , opioid
In this work, various types of metal‐organic frameworks were synthesized, and their affinities toward buprenorphine were evaluated using dispersive solid‐phase extraction. The extracted buprenorphine was determined by ultra high performance liquid chromatography‐ultraviolet detection system. The highest extraction recovery was observed by employing zeolitic imidazole framework‐67. Then, a facile and fast extraction method was designed for the extraction and purification of the target drug. Optimization of the extraction method was carried out by the design of experiment approach. A linearity range of 1–1000 μg/L with the limit of detection of 0.15 μg/L and relative standard deviations (50 μg/L, n = 5) of 3.4% was obtained for standard sample analysis. Under optimized experimental and instrumental conditions, the relative recoveries were in the range of 95 to 111%. Eventually, zeolitic imidazole framework‐67 was successfully employed for the extraction and determination of buprenorphine in the biological fluids with satisfactory results.