Premium
An efficient, green, and easy‐to‐scale‐up strategy for target‐oriented isolating cadinene sesquiterpenoids from Eupatorium adenophorum Spreng
Author(s) -
Zhou Xue,
Wang Jie,
Sun Lifang,
Xiang Anya,
Shi Qinglong,
Li Haichi,
Zhou Dan,
Ge Fahuan
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201901245
Subject(s) - chemistry , chromatography , extraction (chemistry) , distillation , supercritical fluid , supercritical fluid chromatography , eupatorium , high performance liquid chromatography , supercritical fluid extraction , organic chemistry , botany , biology
A green and efficient strategy was established and optimized for target‐oriented extraction, enrichment and separation of cadinene sesquiterpenoids from Eupatorium adenophorum Spreng., using the combination of supercritical fluid extraction, molecular distillation, and industrial preparative chromatography for the first time. The extraction conditions of supercritical fluid extraction were initially optimized by orthogonal experimental design. Under the optimum conditions, the contents of 9‐oxo‐10,11‐dehydroageraphorone and 10 Hβ ‐9‐oxo‐ageraphorone, which were 55.00% and 6.01%, respectively, were much higher than conventional extraction methods. Then, the molecular distillation enrichment method was established and investigated by response surface methodology technology, which showed strong specificity for enriching target compounds and removing impurities from crude extracts. Under the optimum conditions of molecular distillation, total contents of cadinene sesquiterpenoids were increased to 89.19%. Finally, a total of 146 mg of 9‐oxo‐10,11‐dehydroageraphorone and 29 mg of 10 Hβ ‐9‐oxo‐ageraphorone were easily obtained by industrial preparative chromatography, from 200 mg of distillation fraction, with purities over 99%. The contents of target components were analyzed by HPLC, and structures of them were identified by high‐resolution MS, 1 H‐NMR, and 13 C‐NMR spectroscopy. These results indicate that it is a simple, effective, and eco‐friendly strategy, which is easily converted into industrial scale.