Premium
Determination of lipid mediators in breast cancer cells using lyophilization‒supercritical fluid extraction online coupled with supercritical fluid chromatography‒quadrupole tandem mass spectrometry
Author(s) -
Ye Fengying,
Zhong Qisheng,
Liang Yanshan,
Zhou Ting
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201900934
Subject(s) - chromatography , supercritical fluid chromatography , supercritical fluid , chemistry , mass spectrometry , tandem mass spectrometry , supercritical fluid extraction , extraction (chemistry) , liquid chromatography–mass spectrometry , gas chromatography , organic chemistry
A lyophilization‒supercritical fluid extraction coupled with supercritical fluid chromatography‒quadrupole tandem mass spectrometry online method was developed for the determination of lipid mediators in breast cancer cells. Supercritical fluid extraction was applied to the cell samples for the first time due to the use of lyophilization. The conditions of supercritical fluid extraction and supercritical fluid chromatography‒quadrupole tandem mass spectrometry were investigated systematically. Under the optimized conditions, all the calibration curves for the lipid mediators showed good linearity (correlation coefficient > 0.99). The limits of detection and the limits of quantification were in the range of 0.190‒5.36 pg and 0.560‒16.2 pg, respectively. The recoveries were in the range of 70.3‒125%. The relative standard deviations of the precision ranged from 1.49‒18.7% and the accuracies were higher than 84%. Compared with liquid‒liquid extraction coupled with liquid chromatography and tandem mass spectrometry method, the present approach reduced the manual labor and obtained higher sensitivity as well as higher extraction recoveries for all 15 lipid mediators. Finally, the online method was applied to the quantification of lipid mediators in breast cancer cells and normal mammary epithelial cells. On the basis of the results, this lyophilization‒supercritical fluid extraction online coupled with supercritical fluid chromatography‒quadrupole tandem mass spectrometry method showed great promise in the analysis of lipid mediators in complex biological samples.