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Metabolic mapping of Schisandra chinensis lignans and their metabolites in rats using a metabolomic approach based on HPLC with quadrupole time‐of‐flight MS/MS spectrometry
Author(s) -
Wang Jiaye,
Jiang Bin,
Shan Yingying,
Wang Xiaoli,
Lv Xi,
Mohamed Jihan,
Li He,
Wang Chunmei,
Chen Jianguang,
Sun Jinghui
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201900860
Subject(s) - schisandra chinensis , lignan , metabolomics , chemistry , hydroxylation , chromatography , demethylation , metabolite , schisandra , metabolic pathway , tandem mass spectrometry , high performance liquid chromatography , mass spectrometry , biochemistry , metabolism , traditional chinese medicine , medicine , stereochemistry , enzyme , gene expression , alternative medicine , pathology , dna methylation , gene
Schisandra chinensis lignans are the main active components of the traditional Chinese medicine Schisandra chinensis in East Asia. At present, there are more and more medicines and health foods in which the total S. chinensis lignans extracts are considered as the main active components, but little research has been done on the active components of S. chinensis lignans in the blood and main target organs. In this study, the components of S. chinensis lignans in the blood, liver and brain tissues of rats at different time points after the intragastrical administration of S. chinensis lignans were determined by a metabolomic method based on high‐performance liquid chromatography with quadrupole time‐of‐flight tandem mass spectrometry spectrometry. Twelve Schisandra chinensis lignans and 15 metabolites in the blood, liver, and brain of rats were identified. The results showed that the main metabolic ways of S. chinensis lignans in rats were hydroxylation, demethylation, and demethylation‐hydroxylation, and some of them might undergo demethylation, dehydrogenation, epoxidation, and elimination reaction. The time‐dose characteristics of S. chinensis lignans and their metabolites in the blood and target organs were analyzed, which may be helpful to elucidate the active substances that really exert the pharmacodynamic effects of S. chinensis lignans in organisms.