Premium
Microfluidic chip electrophoresis for biochemical analysis
Author(s) -
Ou Xiaowen,
Chen Peng,
Huang Xizhi,
Li Shunji,
Liu BiFeng
Publication year - 2020
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201900758
Subject(s) - microfluidics , capillary electrophoresis , electrophoresis , dielectrophoresis , free flow electrophoresis , chromatography , chemistry , gel electrophoresis , fractionation , nanotechnology , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , materials science , biochemistry , enzyme
Microfluidic chip electrophoresis has been widely employed for separation of various biochemical species owing to its advantages of low sample consumption, low cost, fast analysis, high throughput, and integration capability. In this article, we reviewed the development of four different modes of microfluidics‐based electrophoresis technologies including capillary electrophoresis, gel electrophoresis, dielectrophoresis, and field (electric) flow fractionation. Coupling detection schemes on microfluidic electrophoresis platform were also reviewed such as optical, electrochemical, and mass spectrometry method. We further discussed the innovative applications of microfluidic electrophoresis for biomacromolecules (nucleic acids and proteins), biochemical small molecules (amino acids, metabolites, ions, etc.), and bioparticles (cells and pathogens) analysis. The future direction of microfluidic chip electrophoresis was predicted.