Premium
Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation
Author(s) -
Ramos Yassel,
González Annia,
SosaAcosta Patricia,
PerezRiverol Yasset,
García Yairet,
CastellanosSerra Lila,
Gil Jeovanis,
Sánchez Aniel,
González Luis J.,
Besada Vladimir
Publication year - 2019
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201900495
Subject(s) - chromatography , sodium dodecyl sulfate , chemistry , gel electrophoresis , electroelution , gel electrophoresis of proteins , fractionation , free flow electrophoresis , electrophoresis , polyacrylamide gel electrophoresis , two dimensional gel electrophoresis , capillary electrophoresis , molecular weight size marker , peptide , difference gel electrophoresis , proteomics , biochemistry , gene , enzyme
Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.