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Simultaneous determination of five isoflavones in rat plasma by LC‐MS/MS: Comparative pharmacokinetic characteristics of Puerariae lobatae radix in normal and type 2 diabetic rats
Author(s) -
Zhang Hongmin,
Hu Xintong,
Qiao Miao,
Li Yang,
Cao Shijie,
Ding Liqin,
Feng Xinchi,
Kang Ning,
Zhang Deqin,
Qiu Feng
Publication year - 2019
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201900341
Subject(s) - chromatography , puerarin , chemistry , daidzin , protein precipitation , formic acid , daidzein , pharmacokinetics , genistin , electrospray ionization , radix (gastropod) , analyte , isoflavones , mass spectrometry , genistein , pharmacology , biochemistry , medicine , botany , alternative medicine , pathology , biology
A rapid and sensitive liquid chromatography with tandem mass spectrometry method was developed and validated for simultaneous determination of puerarin, daidzin, daidzein, 3′‐hydroxy puerarin, and genistein in rat plasma after oral administration of Puerariae lobatae radix extract. The method of protein precipitation with acetonitrile was used for sample preparation. Chromatographic separation was achieved on a C 18 column with the mobile phases of acetonitrile/water containing 0.1% formic acid. The analytes were detected by mass spectrometer with an electrospray ionization source operating in the negative ion mode. The linearity, precision, accuracy, dilution reliability, recovery, matrix effects, and stability of the method were within acceptable ranges. The developed method was successfully used to compare the pharmacokinetic characteristics of five analytes in normal and type 2 diabetics rats after oral administration of Puerariae lobatae radix extract. Several pharmacokinetic alterations were observed and this might be caused by the pathological state of type 2 diabetes.