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Exploration and optimization of conditions for quantitative analysis of lignans in Schisandra chinensis by an online supercritical fluid extraction with supercritical fluid chromatography system
Author(s) -
Dai Zhuoshun,
Xin Huaxia,
Fu Qing,
Hao Hongyuan,
Li Qiang,
Liu Qiaoxia,
Jin Yu
Publication year - 2019
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201900222
Subject(s) - schisandra chinensis , chromatography , supercritical fluid , supercritical fluid chromatography , chemistry , supercritical fluid extraction , extraction (chemistry) , elution , quantitative analysis (chemistry) , volumetric flow rate , high performance liquid chromatography , organic chemistry , medicine , physics , alternative medicine , pathology , traditional chinese medicine , quantum mechanics
An online supercritical fluid extraction with supercritical fluid chromatography system could provide sequential extraction and quantitative analysis of lignans in Schisandra chinensis . Supercritical fluid extraction conditions were optimized at 15 MPa, 50°C, and 4 min with supercritical CO 2 adding 1% methanol; the elution volume and flow rate were set at 6 mL and 2 mL/min to blow extract out of the tank completely. The split‐flow rate was confirmed at 2.5%, which determines injection volume and accuracy of quantitative detection. The factors having negative influences on supercritical fluid chromatography retention in the online system, including sample loading forms and backpressure settings, are discussed in the paper. At last, an extraction‐quantitative method for lignans in Schisandra chinensis was developed, which could be finished within 19.5 min. The total content percentage of four lignans (Schisandrin, Schisandrin A, Schisandrin B and Schisandrol B) in four batches was respectively measured to be 1.42, 1.54, 1.62, and 1.90%.