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Sol‐gel technique for the preparation of β ‐cyclodextrin gold nanoparticles as chiral stationary phase in open‐tubular capillary electrochromatography
Author(s) -
Jiang Zhen,
Qu Jialin,
Tian Xiangge,
Huo Xiaokui,
Zhang Jianbin,
Guo Xingjie,
Fang Linlin
Publication year - 2019
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201900071
Subject(s) - capillary electrochromatography , electrochromatography , chromatography , chemistry , capillary action , monolith , monolithic hplc column , cyclodextrin , capillary electrophoresis , high performance liquid chromatography , materials science , composite material , organic chemistry , catalysis
A novel open‐tubular capillary electrochromatography column coated with β ‐cyclodextrin was prepared using the sol‐gel technique. In the sol‐gel approach, owing to the three‐dimensional network of sol‐gel and the strong chemical bond between the stationary phase and the surface of capillary columns, good chromatographic characteristics and unique selectivity in separating enantiomers were shown. The influences of capillary inner diameter, coating time, organic modifier, buffer pH, and buffer concentration on separation were investigated. The sol‐gel‐coated β ‐cyclodextrin column has shown improved enantioseparation efficiency of chlorphenamine, brompheniramine, pheniramine, zopiclone in comparison with the sol‐gel matrix capillary column. The migration time relative standard deviation of the separation of the enantiomers was less than 0.89% over five runs and 2.9% from column to column. This work confirmed that gold nanoparticles are promising electrochromatographic support to enhance the phase ratio of open‐tubular capillary electrochromatography column in capillary electrochromatography.