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Design of selective molecularly imprinted sorbent for the optimized solid‐phase extraction of S ‐pramipexole from the model multicomponent sample of human urine
Author(s) -
Woźnica Marcin,
Luliński Piotr
Publication year - 2019
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201801101
Subject(s) - molecularly imprinted polymer , sorbent , solid phase extraction , chromatography , elution , ethylene glycol dimethacrylate , chemistry , itaconic acid , extraction (chemistry) , molecular imprinting , sample preparation , polymer , adsorption , monomer , organic chemistry , selectivity , methacrylic acid , catalysis
The objective of this article was to design the selective molecularly imprinted sorbent dedicated to the solid‐phase extraction of S ‐pramipexole from the complex matrix such as human urine. For that purpose, S ‐2,6‐diamino‐4,5,6,7‐tetrahydrobenzothiazole was used as the template acting as the structural analog of S ‐pramipexole and five various monomers were employed in the presence of ethylene glycol dimethacrylate to produce molecularly imprinted polymers. The binding capabilities of resulted polymers revealed that the highest imprinting effect was noted for polymer prepared from the itaconic acid. The comprehensive analysis of morphology and the characterization of binding sites showed not only negligible differences in the extension of surfaces of imprinted and nonimprinted polymers but also higher heterogeneity of binding sites in the imprinted material. Comprehensive optimization of the molecularly imprinted solid‐phase extraction allowed to select the most appropriate solvents for loading, washing, and elution steps. Subsequent optimization of mass of sorbent and volumes of solvents allowed to achieve satisfactory total recoveries of S ‐pramipexole from the model multicomponent real sample of human urine that equals to 91.8 ± 3.2% for imprinted sorbent with comparison to only 37.1 ± 1.1% for Oasis MCX.