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Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (2015–mid 2018)
Author(s) -
Štěpánová Sille,
Kašička Václav
Publication year - 2019
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201801090
Subject(s) - capillary electrophoresis , proteomics , mass spectrometry , capillary action , chromatography , chemistry , electromigration , proteome , capillary electrophoresis–mass spectrometry , materials science , electrospray ionization , biochemistry , composite material , gene
This review summarizes recent developments and applications of capillary and microchip electroseparation methods in proteomic and peptidomic analyses since the year 2015 to ca . mid 2018. Sample preparation procedures for the removal of interfering components or for pre‐fractionation and preconcentration of proteins and peptides of interest are discussed. The innovations in coupling of capillary or microchip electroseparation methods with different modes of mass spectrometry detection are covered. In addition, significant recent applications of capillary electromigration methods in both bottom‐up and top‐down proteomics as well as in determinations of post‐translational modifications of proteins are presented. Moreover, several examples of the utilization of capillary electromigration methods coupled with mass spectrometry detection for clinical proteomics and peptidomics are described.