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Determination of residual solvents in isobutylboronic acid: Masking derivatization improves stability of GC column
Author(s) -
Papoušek Roman,
Žák Stanislav,
Štverka Pavel,
Gavenda Aleš,
Borovcová Lucie,
Barták Petr
Publication year - 2019
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800968
Subject(s) - derivatization , chromatography , chemistry , residual , gas chromatography , reagent , derivative (finance) , high performance liquid chromatography , organic chemistry , algorithm , computer science , financial economics , economics
Masking derivatization was introduced for the determination of residual solvents in samples containing a volatile reactive matrix component(s). Isobutylboronic acid, used in the last step of Bortezomib synthesis, represents a compound passing to the gas phase and deteriorating a chromatographic column during a headspace analysis. The masking derivatization with 1,8‐diaminonaphthalene allowed a simple and straightforward conversion of isobutylboronic acid to a stable nonvolatile derivative and thus prevented gas chromatography column deterioration. The method was successfully validated according to the guidelines of International Committee for Harmonization (Q3C (R6) Guideline for Residual Solvents) and international pharmacopoeias (Ph. Eur., USP) and approved by Teva Czech Industries for routine application.