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Study on the metabolites of betulinic acid in vivo and in vitro by ultra high performance liquid chromatography with time‐of‐flight mass spectrometry
Author(s) -
Zhang Wendan,
Jin Miaomiao,
Jiang Honghong,
Yang Jianxi,
Wang Qiao,
Du Yingfeng,
Cao Liang,
Xu Huijun
Publication year - 2019
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800960
Subject(s) - betulinic acid , in vivo , chemistry , metabolite , chromatography , metabolic pathway , mass spectrometry , in vitro , metabolism , biochemistry , biology , genetics , microbiology and biotechnology
Betulinic acid is a triterpenoid organic acid with remarkable antitumor properties and is naturally present in many fruits, condiments and traditional Chinese medicines. Currently, a strategy was developed for the identification of metabolites following the in vivo and in vitro biotransformation of Betulinic acid with rat intestinal bacteria utilizing ultra high performance liquid chromatography with time‐of‐flight mass spectrometry with polymeric solid‐phase extraction. As a result, 46 metabolites were structurally characterized. The results demonstrated that Betulinic acid is universally metabolized in vivo and in vitro, and Betulinic acid could undergo general metabolic reactions, including oxidation, methylation, desaturation, loss of O and loss of CH 2 . Additionally, the main metabolic pathways in vivo and in vitro were determined by calculating the relative content of each metabolite. This is the first study of Betulinic acid metabolism in vivo, whose results provide novel and useful data for better understanding of the safety and efficacy of Betulinic acid.