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Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time
Author(s) -
DoresSousa José Luís,
Vos Jelle,
Eeltink Sebastiaan
Publication year - 2019
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800891
Subject(s) - chromatography , dispersion (optics) , visualization , high performance liquid chromatography , resolution (logic) , column (typography) , chemistry , kinetic energy , analytical chemistry (journal) , process engineering , computer science , data mining , engineering , artificial intelligence , physics , optics , telecommunications , frame (networking) , quantum mechanics
This review describes chromatographic dispersion and different plate‐height models frequently used to assess the chromatographic performance of ultra‐high‐pressure liquid chromatography column technology. Furthermore, different performance indices, including the resolution, the separation impedance, and kinetic plots are discussed allowing to quantify and visualize the resolving power in liquid chromatography. The construction of kinetic plots is explained, and different visualization approaches are highlighted. Finally, key instrument and column‐technology developments to advance the kinetic performance limits are discussed and selected state‐of‐the‐art applications are highlighted.