Premium
Monitoring of cefepime in urine by micellar electrokinetic capillary chromatography with ultraviolet detection and liquid chromatography coupled to mass spectrometry
Author(s) -
Kummer Mirjam,
Šestáková Nela,
Theurillat Regula,
HuynhDo Uyen,
Endimiani Andrea,
Sendi Parham,
Thormann Wolfgang
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800763
Subject(s) - chromatography , cefepime , micellar electrokinetic chromatography , chemistry , mass spectrometry , capillary electrophoresis , liquid chromatography–mass spectrometry , biochemistry , antibiotic resistance , imipenem , antibiotics
Cefepime monitoring in urine by micellar electrokinetic capillary chromatography with UV detection and liquid chromatography coupled to mass spectrometry via electrospray ionization is described. For micellar electrokinetic capillary chromatography, sample preparation comprised urine dilution and dodecyl‐sulfate protein precipitation at pH 4.5, whereas diluted urines were analyzed in the other assay. Both approaches provided suitable conditions for cefepime analysis in urines of healthy volunteers that were spiked with cefepime. Cefepime monitoring by micellar electrokinetic capillary chromatography in samples from patients taking multiple drugs were prone to interferences, whereas liquid chromatography coupled to mass spectrometry provided clean chromatograms and thus selective detection of cefepime in all samples. The latter assay was used to measure urinary cefepime in a prospective pilot study and to assess cefepime stability in urines at 25, 4, −20 and −70°C. The data suggest that urinary cefepime is stable for at least 72 h at all tested temperatures.