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Molecular‐sieve‐based matrix solid‐phase extraction combined with field‐amplified sample stacking in capillary electrophoresis for the determination of three organic acids in a complex solid matrix
Author(s) -
Wei Mengmeng,
Liu Caijing,
Zhang Huan,
Jiang Luyi,
Yan Jizhong,
Chu Chu
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800703
Subject(s) - solid phase extraction , capillary electrophoresis , chromatography , stacking , chemistry , matrix (chemical analysis) , extraction (chemistry) , sieve (category theory) , detection limit , analytical chemistry (journal) , dispersion (optics) , organic chemistry , physics , mathematics , combinatorics , optics
A simple, convenient, and sensitive method that involves combining matrix solid‐phase dispersion extraction with field‐amplified sample stacking in capillary electrophoresis has been developed for the determination of organic acids in a complex solid matrix. Mesoporous molecular sieve, MCM‐48, was synthesized by a hydrothermal method and selected as the adsorbent in matrix solid‐phase dispersion. After fast extraction, the enriched analytes were back‐extracted into a basic aqueous solution for field‐amplified sample stacking in capillary electrophoresis. Parameters that affect extraction efficiency and sample stacking were optimized. Under the optimal conditions, approximately 42‐, 49‐, and 56‐fold sensitivity enhancements were achieved for danshensu, protocatechuic acid, and cinnamic acid, respectively, when compared to normal injection. A satisfactory correlation coefficient ( r > 0.99) was obtained. Both intra‐ and interday precision were lower than 2.53%. And the limits of detection of the three organic acids ranged between 0.01 and 0.029 μg/mL. Finally, the newly proposed method was successfully applied to the determination of organic acids in Fufang Danshen tablets, which indicates its great potential in analyzing organic acids in a complex matrix.