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Monolithic thin‐layer chromatography plates with covalently bonded matrix for hyphenation with matrix‐assisted laser desorption/ionization
Author(s) -
Kucherenko Elizaveta,
Kanateva Anastasiia,
Kurganov Alexander,
Borisov Roman,
Pirogov Andrey
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800679
Subject(s) - glycidyl methacrylate , mass spectrometry , analytical chemistry (journal) , desorption , chromatography , materials science , polymerization , methacrylate , chemistry , matrix assisted laser desorption/ionization , polymer , composite material , adsorption , organic chemistry
Monolithic layers prepared by the copolymerization of ethylene dimethacrylate and glycidyl methacrylate and deposited onto the surface of glass and silicon plates were investigated as thin‐layer chromatography separation media in hyphenated thin‐layer chromatography with matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) analysis. Varying composition of the polymerization mixture and polymerization conditions layers of different porosities and MALDI‐MS compatibility were synthesized. Compatibility with MALDI‐MS was tested using polyethylene glycol and it was demonstrated that layers prepared without glycidyl methacrylate are not compatible with MALDI‐MS and do not allow obtaining any mass spectra. Best results were achieved with layers containing 19 to 29% glycidyl methacrylate. These layers allow the measurement of mass spectra without additional depositing matrix compounds supplying mass spectra almost “clean” in low molar mass range.