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Synthesis and evaluation of a pentafluorobenzamide stationary phase for HPLC separations in the reversed phase and hydrophilic interaction modes
Author(s) -
Ferreira Claudio de Castro,
Gama Mariana Roberto,
da Silva Gisele Silvestre,
Almeida Wanda Pereira,
Collins Carol Hollingworth,
Jardim Isabel Cristina Sales Fontes
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800522
Subject(s) - hydrophilic interaction chromatography , chemistry , phase (matter) , reversed phase chromatography , high performance liquid chromatography , chromatography , infrared spectroscopy , fourier transform infrared spectroscopy , surface modification , spectroscopy , stationary phase , analytical chemistry (journal) , organic chemistry , chemical engineering , physics , quantum mechanics , engineering
A pentafluorobenzamide stationary phase was synthesized by an easy method with no intermediate purification steps. Physicochemical characterization (elemental analysis, fourier transform infrared spectroscopy, 29 Si and 13 C nuclear magnetic resonance spectroscopy) confirmed the presence of pentafluorobenzamide functionalization on the surface of the silica particles. The pentafluorobenzamide stationary phase proved to be quite versatile as it can be used in two different modes in liquid chromatography: reversed phase and hydrophilic interaction liquid chromatography. Chromatographic characterizations in both modes confirmed the multiple interactions established by the new stationary phase, such as hydrogen bonding and π‐π and ion‐exchange interactions. The pentafluorobenzamide stationary phase was successfully employed for the separation of nucleosides and antihypertensive drugs under hydrophilic interaction liquid chromatography conditions, as well as pesticides and benzodiazepine using reversed phase conditions. The stationary phase showed significant potential when compared with commercial columns.