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Diagnostic ion‐oriented identification and simultaneous quantification of chemical components in Allium chinense G. Don
Author(s) -
Qin Zifei,
Lin Pei,
Yao Zhihong,
Chen Zilin,
Yu Yang,
Dai Yi,
He Xiangjiu,
Zhou Hua,
Yao Xinsheng
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800476
Subject(s) - allium , chemistry , quadrupole time of flight , chromatography , allium sativum , tandem mass spectrometry , mass spectrometry , botany , biology
Allium chinense G. Don, a popular edible condiment with reputation of Ganoderma lucidum in vegetables, exerts significant health effects for treating coronary disease but chemical compounds and corresponding contents still remain unclear. In this study, a total of 80 chemical compounds were detected in Allium chinense extracts based on a summarized fragmentation pattern, of which 32 were unambiguously identified with reference standards. Furthermore, a practical and feasible method was developed and validated for simultaneous quantification of 18 chemical compounds, of which 17 compounds were steroidal saponins, by ultra‐high performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry. In addition, it was known that the contents of quantitative compounds varied significantly among multiple Allium chinense samples. Moreover, chemometric analysis results suggested that chinenoside I, macrostemonoside B, and chinenoside II were the most important markers responsible for poor consistency. Taken altogether, this study would be helpful for the chemical authentication and quality control of Allium chinense samples.