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Graphene‐coated magnetic‐sheet solid‐phase extraction followed by high‐performance liquid chromatography with fluorescence detection for the determination of aflatoxins B 1 , B 2 , G 1 , and G 2 in soy‐based samples
Author(s) -
Nouri Nina,
Sereshti Hassan,
Farahani Ali
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800471
Subject(s) - chromatography , sorbent , solid phase extraction , detection limit , extraction (chemistry) , desorption , magnetic nanoparticles , materials science , analytical chemistry (journal) , adsorption , ionic strength , aflatoxin , graphene , solvent , sample preparation , chemistry , nanoparticle , nanotechnology , aqueous solution , organic chemistry , food science
A new method named graphene‐coated magnetic‐sheet solid‐phase extraction based on a magnetic three‐dimensional graphene sorbent was developed for the extraction of aflatoxins prior to high‐performance liquid chromatography with fluorescence detection. The use of a perforated magnetic‐sheet for fixing the magnetic nanoparticles is a new feature of the method. Hence, the adsorbent particles can be separated from sample solution without using an external magnetic field. This made the procedure very simple and easy to operate so that all steps of the extraction process (sample loading, washing, and desorption) were carried out continuously using two lab‐made syringe pumps. The factors affecting the performance of extraction procedure such as the extraction solvent, adsorbent dose, sample loading flow rate, ionic strength, pH, and desorption parameters were investigated and optimized. Under the optimal conditions, the obtained enrichment factors and limits of detection were in the range of 205–236 and 0.09–0.15 μg/kg, respectively. The relative standard deviations were <3.4 and 7.5% for the intraday ( n = 6) and interday ( n = 4) precisions, respectively. The developed method was successfully applied to determine aflatoxins B 1 , B 2 , G 1 , and G 2 in different soy‐based food samples.