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Detection of biogenic amines in pet food ingredients by RP‐HPLC with automated dansyl chloride derivatization
Author(s) -
Learey Jessica J.,
CrawfordClark Shona,
Bowen Benjamin J.,
Barrow Colin J.,
Adcock Jacqui L.
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800455
Subject(s) - dansyl chloride , cadaverine , biogenic amine , chemistry , tryptamine , putrescine , chromatography , derivatization , tyramine , detection limit , high performance liquid chromatography , analyte , spermidine , food spoilage , organic chemistry , biochemistry , enzyme , serotonin , genetics , receptor , biology , bacteria
The detection of biogenic amines is of significant interest to the food industry, as they can be used as indicators of food spoilage and they are potentially toxic. Because of their importance, there is a need for automated methods suitable for industry use that can detect a wide range of biogenic amines at sufficient levels for food analysis. In this work, optimized conditions for the automated determination of biogenic amines (histamine, putrescine, cadaverine, spermine, spermidine, tyramine, and tryptamine) derivatized with dansyl chloride are presented. Limits of detection below 0.2 ppm were achieved for seven biogenic amines and percentage recoveries were between 80 and 109% for the seven analytes spiked into meat meal samples. The method is simple and compared well to an existing method for the detection of biogenic amines in pet food ingredients.