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Rapid quantification of aloin A and B in aloe plants and aloe‐containing beverages, and pharmaceutical preparations by microchip capillary electrophoresis with laser induced fluorescence detection
Author(s) -
Xiao MengWei,
Bai XiaoLin,
Liu YiMing,
Yang Li,
Hu YaDong,
Liao Xun
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800338
Subject(s) - capillary electrophoresis , chromatography , chemistry , anthraquinones , fluorescence , botany , biology , physics , quantum mechanics
A microchip capillary electrophoresis coupled with laser induced fluorescence detection method for the fast determination of aloin was developed and comprehensively applied for the quantification of aloin A and B present in seven aloe plant species, 42 aloin‐containing crude drugs, ten aloe pharmaceutical preparations, and four aloe gel‐containing functional foods. The excitation and emission wavelengths for detection of both aloins were set at 473 and 520 nm, respectively. Sample analysis on a 35 mm length of glass microchip channel was completed within 40 s. An interference study indicated that the other main anthraquinones present in the samples did not interrupt with the target aloins detection, demonstrating the good selectivity of this method. It is demonstrated that this method is fast, facile, and specific for determination of aloin A and B from matrix samples which can be applied to the quality control of a wide varieties of aloe species and aloe‐derived products.