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Chemometrics‐assisted liquid chromatography with full scan mass spectrometry for the interference‐free determination of glucocorticoids illegally added to face masks
Author(s) -
Long WanJun,
Wu HaiLong,
Wang Tong,
Xie LiXia,
Hu Yong,
Fang Huan,
Cheng Li,
Ding YuJie,
Yu RuQin
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800333
Subject(s) - chromatography , chemometrics , chemistry , analyte , detection limit , mass spectrometry , elution , calibration , liquid chromatography–mass spectrometry , analytical chemistry (journal) , mathematics , statistics
A smart chemometrics‐assisted strategy that combines the full scan mode of liquid chromatography with mass spectrometry with second‐order calibration method based on alternating trilinear decomposition algorithm was developed for the rapid determination of 15 glucocorticoids including the epimers betamethasone and dexamethasone illegally added into face masks. Fifteen glucocorticoids were rapidly eluted (11 min) under a simple elution program. By means of the second‐order calibration method, 15 target analytes were successfully quantified in the presence of peak overlaps, unknown interferences and baseline drifts. Notably, the epimers, namely, betamethasone and dexamethasone, were simultaneously quantified by the proposed method under a simple elution program. The average spiked recoveries for all target analytes ranged from 87.3 ± 2.2 to 119.4 ± 5.8%. The validation parameters including sensitivity, selectivity, limit of detection, limit of quantitation, and precision were calculated to validate the accuracy of the proposed method, and the quantitative analysis results were further confirmed by liquid chromatography with tandem mass spectrometry. All results proved that the proposed chemometrics‐assisted liquid chromatography with mass spectrometry strategy was an accurate and fast method to determine epimers and multiple glucocorticoids in complex face mask samples.