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Hydrophobic ionic liquid modified thermoresponsive molecularly imprinted monolith for the selective recognition and separation of tanshinones
Author(s) -
Tang Weiyang,
Row Kyung Ho
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800329
Subject(s) - monolith , monolithic hplc column , molecularly imprinted polymer , ionic strength , detection limit , chromatography , ionic liquid , poly(n isopropylacrylamide) , monomer , chemistry , lower critical solution temperature , fourier transform infrared spectroscopy , elution , chemical engineering , polymer , materials science , high performance liquid chromatography , copolymer , aqueous solution , selectivity , organic chemistry , engineering , catalysis
A hydrophobic ionic liquid modified thermoresponsive molecularly imprinted monolith was synthesized using N ‐isopropylacrylamide as a thermoresponsive monomer and a long‐chain hydrophobic ionic liquid as an auxiliary modification monomer. The ionic‐liquid‐modified thermoresponsive molecularly imprinted polymer was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. When the column temperature was 50°C, the synthesized monolithic column was successfully applied to the selective separation of homologue tanshinones within 7 min and eluted only by water (mobile phase) (theoretical plates more than 1.00 × 10 5 per meter). The negative Gibbs free energy (≤–2.37) values showed that the transfer of the tanshinones from the mobile phase to the stationary phase on this monolithic column was a thermodynamically spontaneous process. Good linearity of the five tanshinones by thermoresponsive monolith was obtained in the range of 0.100–25.0 μg/mL. The limit of detection (S/N = 3) and limit of quantitation (S/N = 10) were less than 0.0390 and 0.0630 μg/mL, respectively, with a relative standard deviation of <4.8%. In this proposed thermoresponsive chromatography method, the separation of homologue analytes can be achieved by changing the column temperature, and the use of water as the mobile phase would decrease the economic cost and organic pollution.