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High‐resolution capillary zone electrophoresis for transferrin glycoform analysis associated with congenital disorders of glycosylation
Author(s) -
Tobler Micha,
Caslavska Jitka,
Burda Patricie,
Thormann Wolfgang
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800082
Subject(s) - capillary electrophoresis , transferrin , isoelectric focusing , glycosylation , chemistry , electrophoresis , chromatography , gel electrophoresis , high resolution , biochemistry , enzyme , remote sensing , geology
High‐resolution capillary zone electrophoresis is used to assess the transferrin profile in serum of patients with eight different congenital disorders of glycosylation that represent type I, type II, and mixed type I/II disorders. Capillary zone electrophoresis data are compared to patterns obtained by gel isoelectric focusing. The high‐resolution capillary zone electrophoresis method is shown to represent an effective tool to assess the diversity of transferrin patterns. Hypoglycosylated disialo‐, monosialo‐, and asialo‐transferrin in type I cases can be distinguished from the corresponding underdesialylated transferrin glycoforms present in type II disorders. The latter can be separated from and detected ahead of their corresponding hypoglycosylated forms of type I patients. Both types of glycoforms are detected in sera of mixed type I/II patients. The assay has the potential to be used as screening method for congenital disorders of glycosylation. It can be run with a few microliters of serum when microvials are used.