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Rapid screening and identification of antioxidants in the leaves of Malus hupehensis using off‐line two‐dimensional HPLC–UV–MS/MS coupled with a 1,1′‐diphenyl‐2‐picrylhydrazyl assay
Author(s) -
Liu Minzhuo,
Huang Xueqian,
Liu Qi,
Chen Miao,
Liao Sen,
Zhu Fawei,
Shi Shuyun,
Yang Hua,
Chen Xiaoqing
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201800007
Subject(s) - flavonols , chromatography , flavones , chemistry , malus , mass spectrometry , high performance liquid chromatography , antioxidant , flavonoid , botany , organic chemistry , biology
The leaves of Malus hupehensis have a strong antioxidant activity and are commonly consumed as a healthy tea. However, detailed information about its antioxidants is incomplete. Herein, we developed an effective strategy based on combining off‐line two‐dimensional high‐performance liquid chromatography with ultraviolet and tandem mass spectrometry detection with a 1,1′‐diphenyl‐2‐picrylhydrazyl assay to rapidly screen and identify the antioxidants from the leaves of M. hupehensis . In the orthogonal two‐dimensional liquid chromatography system, a Venusil HILIC column was used for the first dimension, while a Universil XB‐C 18 column was installed in the second dimension. As a result, 32 antioxidants, including ten dihydrochalcones, two flavanones, nine flavonols, four flavones, and seven phenolic acids were tentatively identified, out of which 23 compounds, as far as we know, were isolated and characterized from the leaves of M. hupehensis for the first time. To the best of our knowledge, this is the first systematic investigation of the antioxidants from the leaves of M. hupehensis . The results indicated that the proposed method is an efficient technique to rapidly investigate antioxidants, especially for coeluted and minor compounds in a complex system.