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Front Cover: Enhanced binding by dextran‐grafting to Protein A affinity chromatographic media
Author(s) -
Zhao Lan,
Zhu Kai,
Huang Yongdong,
Li Qiang,
Li Xiunan,
Zhang Rongyue,
Su Zhiguo,
Wang Qibao,
Ma Guanghui
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201770071
Subject(s) - dextran , chemistry , agarose , chromatography , affinity chromatography , protein a , matrix (chemical analysis) , molecule , antibody , biochemistry , organic chemistry , immunology , biology , enzyme
J. Sep. Sci. 2017, 40 , 1493–1499 DOI: 10.1002/jssc.201601196 The cover picture shows the binding of antibody molecules to dextran‐grafted Protein A affinity chromatographic medium. On the left, a schematic diagram of dextran‐grafted Protein A affinity chromatographic medium is depicted, showing antibody molecules (yellow) binding to Protein A ligands (green) coupled site‐directed to dextran (dark blue)‐grafted agarose‐based matrix (light blue). On the top of the right, confocal laser scanning microscopy images illustrate that immunoglobulin G molecules entered in the medium completely. At the bottom of the right, an atom force microscopy image of dextran‐grafted medium is presented, showing a very rough surface to support the binding results.