Premium
Simultaneous analysis of tropane alkaloids in teas and herbal teas by liquid chromatography coupled to high‐resolution mass spectrometry (Orbitrap)
Author(s) -
RomeraTorres Ana,
RomeroGonzález Roberto,
Martínez Vidal José Luis,
Garrido Frenich Antonia
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201701485
Subject(s) - tropane , chemistry , chromatography , orbitrap , formic acid , datura stramonium , extraction (chemistry) , mass spectrometry , solid phase extraction , resolution (logic) , alkaloid , stereochemistry , botany , artificial intelligence , computer science , biology
A new method has been developed for the simultaneous determination of 13 tropane alkaloids in tea and herbal teas using high‐performance liquid chromatography coupled to an Exactive‐Orbitrap analyzer. A mixture of methanol, water, and formic acid was used for the extraction of the target compounds followed by a solid‐phase extraction step. The validated method provided recoveries from 75 to 128% with intra‐ and interday precision lower than or equal to 24% (except for apoatropine). Limits of quantification ranged from 5 to 20 μg/kg. Eleven tea and herbal tea samples and two contaminated samples with Datura stramonium seeds were analyzed. Tropane alkaloids were detected in six samples with concentrations from 5 (apoatropine) to 4340 μg/kg (sum of physoperuvine, pseudotropine, and tropine), whereas concentrations from 5 (apoatropine) to 1725 μg/kg (sum of physoperuvine, pseudotropine, and tropine) were found in the contaminated samples.