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Adsorbed hollow fiber‐based biological fingerprinting for the discovery of platelet aggregation inhibitors from Danshen–Honghua decoction
Author(s) -
Zhang Qian,
Tan ChengNing,
Wang Yali,
Liu WenJing,
Yang FengQing,
Chen Hua,
Xia ZhiNing
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201701434
Subject(s) - decoction , chemistry , repeatability , chromatography , platelet aggregation , fiber , platelet activation , platelet , traditional medicine , organic chemistry , medicine , immunology , biology
For lead compound discovery from natural products, hollow fiber cell fishing with chromatographic analysis is a newly developed method. In this study, an adsorbed hollow fiber‐based biological fingerprinting method was firstly developed to discover potential platelet aggregation inhibitors from Danshen–Honghua decoction. Platelets were seeded on the fiber and their survival rate was tested. Results indicated that more than 92% platelets survived during the whole operation process. Ranitidine and tirofiban were used as positive and negative control respectively to verify the reliability of the presented approach. The main variables such as amount of extract and stirring time that affect the adsorbed hollow fiber‐based biological fingerprinting process were optimized, and the repeatability of this method was also investigated. Finally, 12 potential active compounds in Danshen–Honghua decoction were successfully detected using the established approach and structures for nine of them were tentatively identified by liquid chromatography with mass spectrometry analysis. In addition, the in vitro platelet aggregation inhibition test was carried out for five of the nine hit compounds, and three active components, namely, lithospermic acid, salvianolic acid A, and salvianolic acid B were confirmed. These results proved that the proposed method could be an effective approach for screening platelet inhibitors from plant extracts.