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A convenient ultrasound‐assisted saponification for the simultaneous determination of vitamin E isomers in vegetable oil by HPLC with fluorescence detection
Author(s) -
Yang Yi,
Lu Dan,
Yin Shuo,
Yang Danni,
Chen Yaling,
Li Yongxin,
Sun Chengjun
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201701393
Subject(s) - saponification , chromatography , chemistry , potassium hydroxide , detection limit , analyte , vegetable oil , fluorescence spectroscopy , high performance liquid chromatography , fluorescence , methanol , analytical chemistry (journal) , organic chemistry , physics , quantum mechanics
An efficient ultrasound‐assisted saponification was developed for simultaneous determination of vitamin E isomers in vegetable oil by high‐performance liquid chromatography with fluorescence detection. The samples were saponified ultrasonically with potassium hydroxide solution for only 7 min, then the analytes were extracted with ether. Vitamin E isomers were separated on a C 18 column at 25°C with a mobile phase of methanol/acetonitrile (81:19, v/v) at a flow rate of 0.8 mL/min. Fluorescence detection was operated at 290 nm of excitation wavelength and 327 nm of emission wavelength. Under the optimized conditions, good linearities over the range of 0.001–8.00 μg/mL ( r > 0.999) were obtained. Mean recoveries of the method were 88.0–106%, with intra‐ and interday RSDs less than 11.8 and 12.8%, respectively. The detection limits and quantification limits of the method were 0.30–1.8 and 1.0–6.1 μg/kg, respectively. The recoveries of this method were much higher than that of the quick, easy, cheap, effective, rugged, and safe method and direct dilution method, but were similar to those of hot saponification. This proposed method provides reliable, simple, and rapid quantification of vitamin E isomers in vegetable oils. Five kinds of vegetable oils were analyzed, the quantification results were within the ranges reported by other authors.