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Simultaneous determination of enantiomers of carfentrazone‐ethyl and its metabolite in eight matrices using high‐performance liquid chromatography with tandem mass spectrometry
Author(s) -
Duan Jinsheng,
Dong Xu,
Shen Yang,
Gao Beibei,
Zhang Zhaoxian,
Gao Tongchun,
Wang Minghua
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201701349
Subject(s) - chemistry , chromatography , enantiomer , metabolite , stereochemistry , biochemistry
A reversed‐phase simultaneous determination method for the enantiomers of carfentrazone‐ethyl and its metabolite carfentrazone in agricultural and environmental samples was established using high‐performance liquid chromatography‐tandem mass spectrometry. A complete enantioseparation of carfentrazone‐ethyl and its chiral metabolite carfentrazone enantiomers was obtained using a chiral column. The absolute configuration and specific optical rotation of carfentrazone‐ethyl and carfentrazone enantiomers were first confirmed as peaks 1, 2, 3, and 4: S ‐(+)‐carfentrazone, R ‐(‐)‐carfentrazone, S ‐(‐)‐carfentrazone‐ethyl, and R ‐(+)‐carfentrazone‐ethyl, respectively. The specificity, matrix effect, linearity, precision, accuracy, and stability were surveyed to evaluate the feasibility of the method. The mean recovery was in the range of 77.5–102.8% with relative standard deviations of 0.4‐9.8% for the samples. The limits of detection of the enantiomers were evaluated as 0.7 to 6.0 μg/kg, and the limits of quantification were 2.5 to 20 μg/kg. The stereoselective degradation of carfentrazone‐ethyl and carfentrazone in rice plant was investigated, and there was no clear enantioselectivity for carfentrazone‐ethyl. As for carfentrazone, the enantiomer fractions value reached 0.85 at 7 days after spraying. The developed method was simple and reliable enough for the corresponding risk assessment of carfentrazone‐ethyl and its metabolite enantiomers in crop plants, cereal grains, and soil samples.