Premium
Flotation/ultrasound‐assisted microextraction followed by HPLC for determination of fat‐soluble vitamins in multivitamin pharmaceutical preparations
Author(s) -
Shahdousti Parvin,
Aghamohammadi Mohammad
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201701347
Subject(s) - multivitamin , chromatography , chemistry , high performance liquid chromatography , fat soluble vitamin , vitamin , biochemistry
Dissolved carbon dioxide flotation–emulsification microextraction technique coupled with high‐performance liquid chromatography was developed for separation and determination of fat‐soluble vitamins (A, D 3 , E, and K 3 ) in multivitamin pharmaceutical preparations. Dissolved carbon dioxide flotation was used to break up the emulsion of extraction solvent in water and to collect the extraction solvent on the surface of aqueous sample in narrowed capillary part of extraction cell. Carbon dioxide bubbles were generated in situ through the addition of 300 μL of concentrated hydrochloric acid into the alkaline sample solution at pH = 11.5 (1% w/v sodium carbonate), which was sonicated to intensify the carbon dioxide bubble generation. Several factors affecting the extraction process were optimized. Under the optimal conditions, the limits of detection were 0.11, 0.47, 0.20 and 0.35 μg/L for A, E, D 3 , and K 3 vitamins in water samples, respectively. The inter‐day and intra‐day precision of the proposed method were evaluated in terms of the relative standard deviation and were <10.5%.