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Separation of three polar compounds from Rheum tanguticum by high‐speed countercurrent chromatography with an ethyl acetate/glacial acetic acid/water system
Author(s) -
Chen Tao,
Wang Ping,
Wang Nana,
Sun Chongyang,
Yang Xue,
Li Hongmei,
Zhou Guoying,
Li Yulin
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201701298
Subject(s) - countercurrent chromatography , chromatography , chemistry , acetic acid , ethyl acetate , gallic acid , column chromatography , organic chemistry , antioxidant
The separation of polar compounds by high‐speed countercurrent chromatography is still regarded as a challenge. In this study, an efficient strategy for the separation of three polar compounds from Rheum tanguticum has been successfully conducted by using high‐speed countercurrent chromatography. X‐5 macroporous resin chromatography was used for the fast enrichment of the target compounds. Then, the target fraction was directly introduced into high‐speed countercurrent chromatography for separation using ethyl acetate/glacial acetic acid/water (100:1:100, v/v/v) as the solvent system. Consequently, three polar compounds including gallic acid, catechin, and gallic acid 4‐ O ‐β‐ d ‐(6′‐ O ‐galloyl) glucoside were obtained with purities higher than 98%. The results showed glacial acetic acid could be such an appropriate regulator for the ethyl acetate/water system. This study provides a reference for the separation of polar compounds from natural products by high‐speed countercurrent chromatography.