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Isolation of sinapic acid from broccoli using molecularly imprinted polymers
Author(s) -
Hosny Heba,
El Gohary Nesrine,
Saad Engy,
Handoussa Heba,
El Nashar Rasha M.
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201701120
Subject(s) - molecularly imprinted polymer , ethylene glycol dimethacrylate , polymer , chemistry , monomer , polymerization , organic chemistry , molecular imprinting , chromatography , combinatorial chemistry , methacrylic acid , selectivity , catalysis
A molecularly imprinted polymer was synthesized for the purpose of sinapic acid isolation from Egyptian nutraceutical Botrytis italica , L. (broccoli) due to its prominent medicinal and wide pharmacological activities. A computational study was first developed to determine the optimal template to functional monomer molar ratio. Based on the computational results, five polymers were synthesized using a bulk polymerization method with sinapic acid as the template molecule. Evaluation of the synthesized polymers binding performance was carried out using batch rebinding assay, which revealed that the molecularly imprinted polymer of molar ratio (1:4:20), template to functional monomer (4‐vinyl pyridine) to crosslinker (ethylene glycol dimethacrylate) was of optimum performance, thus, this polymer was applied for sinapic acid isolation from closely related analogues. This represents a more practical approach to isolate sinapic acid from different natural extracts selectively.