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Modified dispersive liquid‐phase microextraction based on sequential injection solidified floating organic drop combined with HPLC for the determination of phenobarbital and phenytoin
Author(s) -
Amiri Pebdani Arezou,
Dadfarnia Shayessteh,
Haji Shabani Ali Mohammad,
Khodadoust Saeid,
Talebianpoor Mohammad Sharif
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201701111
Subject(s) - phenobarbital , chromatography , detection limit , chemistry , calibration curve , phenytoin , extraction (chemistry) , high performance liquid chromatography , analytical chemistry (journal) , pharmacology , medicine , neuroscience , epilepsy , biology
A modified dispersive liquid phase microextraction based on sequential injection solidified floating organic drop was developed for simultaneous separation/preconcentration of trace amounts of phenobarbital and phenytoin. The important factors affecting on the extraction recovery including pH, the volume of extraction solvent, ionic strength, and the number of injections were investigated and optimized by Box–Behnken design and desirability function. Under the optimum experimental conditions, the calibration graph was linear in the concentration range of 1.0–300.0 μg/L ( r 2 = 0.997) for phenobarbital and 2.0–400.0 μg/L ( r 2 = 0.996) for phenytoin. The limit of detection and limit of quantification were 0.35 and 1.2 μg/L for phenobarbital and 0.65 and 2.2 μg/L for phenytoin, respectively. The relative standard deviation for six replicate determinations at 10 μg/L was 3.3 and 4.1% for phenobarbital and phenytoin, respectively. The developed method was successfully applied to the determination of phenobarbital and phenytoin in urine and plasma samples.