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Magnetic separation coupled with high‐performance liquid chromatography–mass spectrometry for rapid separation and determination of lignans in Schisandra chinensis
Author(s) -
Piao Jishou,
Liu Lu,
Wang Sihong,
Shang HaiBo,
He Miao,
Quan Ninghai,
Li Donghao
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201701098
Subject(s) - schisandra chinensis , polyethylenimine , chromatography , superparamagnetism , chemistry , magnetic separation , magnetic nanoparticles , mass spectrometry , detection limit , analytical chemistry (journal) , fourier transform infrared spectroscopy , nanoparticle , materials science , nanotechnology , magnetization , chemical engineering , magnetic field , alternative medicine , transfection , pathology , engineering , biochemistry , traditional chinese medicine , quantum mechanics , metallurgy , medicine , physics , gene
Despite the strong antihepatotoxic, antioxidant, and antitumor properties of lignans from Schisandra chinensis , their applications in new drug development, bioscience and functional foods, etc. are limited because of their low abundance and complex coextractions. In this study, a magnetic separation method has been developed based on polyethylenimine‐modified magnetic nanoparticles to rapidly and effectively separate and purify the lignans from S. chinensis crude extracts through cation–π interaction and electrostatic adsorption. The magnetic nanoparticles were characterized by transmission electron microscopy, vibrating sample magnetometry, Fourier transform infrared spectroscopy, and X‐ray diffraction. Polyethylenimine‐modified magnetic nanoparticles showed a spherical‐shaped morphology and the average size was about 10 nm with superparamagnetism. Under the pH 7.4, polyethylenimine modified magnetic nanoparticles can remove a lot of coextracts. The range of detection limits and quantification limits was 0.27–0.34 and 0.89–1.13 ng/mL, respectively. Compared with other common methods, the magnetic separation method proposed in this study is much simpler and more effective through both strong cation–π interaction and electrostatic interaction.