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Rapid analysis of traditional Chinese medicine Pinellia ternata by microchip electrophoresis with electrochemical detection
Author(s) -
Shih TsungTing,
Lee HuiLing,
Chen ShowChuen,
Kang ChihYuan,
Shen RenShang,
Su YiAn
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700901
Subject(s) - pinellia ternata , chromatography , chemistry , pinellia , traditional medicine , traditional chinese medicine , medicine , alternative medicine , pathology
Traditional Chinese herbal medicine has long enjoyed the reputation of the world's most advanced system of natural medicine. Pinellia ternata is one of the most commonly used herbs in the traditional Chinese medical science. In this study, five representative ingredients of Pinellia ternata guanosine, methionine, glycine, 3,4‐dihydroxybenzaldehyde, and homogentisic acid, were assayed using simple derivatization procedures. Under optimized experimental condition, five analytes in Pinellia ternata were rapidly separated and detected using microchip electrophoresis, affording the benefits of speed, minimal sample requirements, and sensitive on‐the‐chip electrochemical detection, in 5 min with linearity over a concentration of 20–500 μM ( R 2 = 0.994) with nearly complete recovery (95.6–98.5%).