Premium
Liquid chromatography coupled with hybrid ion trap time‐of‐flight mass spectrometry method for the determination of caulophine in rat bio‐samples and its pharmacokinetic study after intragastric and intraperitoneal administration
Author(s) -
Lu Dan,
Xu Xiao,
Li Chunlei,
Wang Sicen
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700846
Subject(s) - chromatography , chemistry , protein precipitation , mass spectrometry , electrospray ionization , formic acid , extraction (chemistry) , ion trap , electrospray , calibration curve , analytical chemistry (journal) , detection limit
A rapid and precise liquid chromatography coupled with hybrid ion trap/time‐of‐flight mass spectrometry method to detect and quantify caulophine and its possible active metabolites in rat plasma and urine was developed. Samples were prepared by plasma protein precipitation combined with a liquid‐liquid extraction method. The separation was carried out on an InertSustain® C18 column with a mobile phase comprising methanol and 0.1% aqueous formic acid solution. The analysis was complete in 20 min with a flow rate of 0.4 mL/min. Taspine was used as the internal standard. Mass spectrometric detection was conducted with hybrid ion trap/time‐of‐flight equipped with electrospray ionization in the positive ion mode. The calibration curves of caulophine were linear over the concentration ranges of 0.002–0.20 μg/mL for plasma and 0.005–0.50 μg/mL for urine with the correlation coefficients greater than 0.998 in both cases. The method was successfully used to investigate the pharmacokinetics and bioavailability in rat plasma and urine samples after intragastric and intraperitoneal administration of caulophine sodium salt.