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Rapid characterization of the absorbed chemical constituents of Tangzhiqing formula following oral administration using UHPLC–Q‐TOF‐MS
Author(s) -
Wu XiaoLei,
Wu MingJiang,
Chen XinZe,
Zhang HongMin,
Ding LiQin,
Tian FuYing,
Fu XiaoMeng,
Qiu Feng,
Zhang DeQin
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700777
Subject(s) - sulfation , chemistry , glucuronidation , chromatography , demethylation , oral administration , chemical constituents , mass spectrometry , urine , pharmacology , organic chemistry , biochemistry , medicine , gene expression , dna methylation , gene , enzyme , microsome
Tangzhiqing formula, a Chinese herbal formula, is used for the treatment of type II diabetes and prediabetes. Although its effectiveness has been certified by clinical use, its absorbed chemical constituents are not comprehensively represented. Thence, in order to reveal potential bioactive components and metabolism of Tangzhiqing formula, an ultra‐high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry method was developed. A total of 86 absorbed components, including 38 prototype compounds and 48 metabolites, were identified in rat plasma, urine, and feces after oral administration of Tangzhiqing formula. This was the first systematic study on the chemical constituents and metabolic profiling of Tangzhiqing formula. The results indicated that alkaloids and flavonoids were main absorbed components, and glucuronidation and sulfation were the major metabolites. Moreover we concluded that alkaloids and flavonoids first underwent demethylation and hydrolysis reactions before biotransformed to phase II metabolites. This study provided valuable data for safety estimation of Tangzhiqing formula, which will be advantageous for clinical application.