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Preparation of a long‐alkyl‐chain‐based hybrid monolithic column with mixed‐mode interactions using a “one‐pot” process for pressurized capillary electrochromatography
Author(s) -
Lyu Haixia,
Zhao Heqing,
Qin Wenfei,
Xie Zenghong
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700772
Subject(s) - capillary electrochromatography , monolith , azobisisobutyronitrile , monolithic hplc column , chemistry , monomer , alkyl , chromatography , electrochromatography , chemical engineering , high performance liquid chromatography , organic chemistry , capillary electrophoresis , polymer , catalysis , engineering
A simple “one‐pot” approach for the preparation of a new vinyl‐functionalized organic–inorganic hybrid monolithic column is described. In this improved method, the hydrolyzed alkoxysilanes of tetramethoxysilane and triethoxyvinylsilane were used as precursors for the synthesis of a silica‐based monolith, while 1‐hexadecene and sodium ethylenesulfonate were used as vinyl functional monomers along with azobisisobutyronitrile as an initiator. The effects of reaction temperature, urea content, and composition of organic monomers on the column properties (e.g. morphology, mechanical stability, and chromatographic performance) were investigated. The monolithic column was used for the separation of neutral solutes by reversed‐phase pressurized capillary. Furthermore, the monolith can separate various aromatic amines, which indicated its excellent cation‐exchange capability and hydrophobic interactions. The baseline separation of the aromatic amines was obtained with a column efficiency of up to 78 000 plates/m.