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High‐performance liquid chromatography thermodynamic study of new potential antiepileptic compounds on a cholesterol column using isocratic elution with methanol/water and acetonitrile/water eluent systems
Author(s) -
Flieger Jolanta,
Trębacz Hanna,
Pizoń Magdalena,
Kowalska Anna,
Szczęsna Agnieszka,
Plech Tomasz
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700748
Subject(s) - chemistry , gibbs free energy , enthalpy , elution , differential scanning calorimetry , chromatography , column chromatography , phase (matter) , reversed phase chromatography , standard molar entropy , high performance liquid chromatography , thermodynamics , standard enthalpy of formation , organic chemistry , physics
Basic thermodynamic functions responsible for retention of new 1,2,4‐triazole derivatives exhibiting varied antiepileptic activity on cholesterol‐based stationary phase were determined. Evaluation of the Gibbs energy change, the change in enthalpy and the change in entropy was based on the van't Hoff relationship representing ln k versus 1/ T . A detailed discussion of the van't Hoff equation, exploring the influence of the phase ratio on deviations from linearity in a van't Hoff plot is presented. We show chromatographic evidence to the question of how a varied mobile phase composition may cause different thermodynamic phase ratios. The analysis of data from a differential scanning calorimetry excluded any phase transitions of either the individual solutes or cholesterol stationary phase suspended in the mobile phase components within the studied temperature range.