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Simultaneous analysis of carbohydrates, polyols and amines in urine samples using chemical ionization gas chromatography with tandem mass spectrometry
Author(s) -
Kubinec Róbert,
Kotora Peter,
Ferenczy Viktória,
Blaško Jaroslav,
Podolec Peter,
Hengerics Szabó Alexandra,
Behúlová Darina,
Bierhanzl Václav,
Čabala Radomír,
Stuchlík Stanislav,
Filipiak Wojciech,
Thắng Ngô Mạnh
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700715
Subject(s) - derivatization , chemistry , chromatography , chemical ionization , mass spectrometry , gas chromatography/tandem mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , trimethylsilyl , galactosemia , tandem mass spectrometry , urine , sample preparation , organic chemistry , ionization , galactose , ion , biochemistry
A simple method for the simultaneous derivatization of carbohydrates, polyols, amines and amino acids using hexamethyldisilazane and N , O ‐bis(trimethylsilyl)trifluoroacetamide was developed. This method allows the direct derivatization of urine samples without sample pretreatment before derivatization. The method was successfully used for analysis of the selected metabolites in urine samples of healthy individuals and neonates suffering from galactosemia. The limits of detection by positive chemical ionization gas chromatography with tandem mass spectrometry analysis were in the range of 1.0 mgL ‐1 for mannitol to 4.7 mg/L for glucose.