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Qualitative and quantitative analysis of chemical constituents in Ardisiae Japonicae Herba
Author(s) -
Yu KeYun,
Gao Wen,
Li ShangZhen,
Wu Wei,
Li Pei,
Dou LiLi,
Wang YuanZhong,
Liu EHu
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700667
Subject(s) - chromatography , chemistry , quercitrin , repeatability , mass spectrometry , chemical constituents , quadrupole time of flight , bergenin , phenylethanoid , glycoside , electrospray ionization , flavonoid , organic chemistry , antioxidant
Ardisiae Japonicae Herba is a well‐known traditional Chinese medicine for the treatment of bronchitis conjunctivitis, pneumonia, and trauma. In this work, a high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry method was first established for the separation and structural identification of the chemical constituents in Ardisiae Japonicae Herba. A total of 15 compounds including coumarins, flavonoid glycosides, and catechins were identified or tentatively characterized based on their chromatographic behaviors and mass spectral fragmentation and by comparisons with the reference standards. Furthermore, a simple high‐performance liquid chromatography with diode array detection method was developed for the simultaneous determination of five major constituents. Results obtained from method validation, including linearity, precision, repeatability, stability, and recovery, showed that the established method was reliable and accurate. Bergenin and quercitrin were found to be the most abundant constituents and could be served as chemical markers for quality control of Ardisiae Japonicae Herba.