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Systematic study on metabolism and activity evaluation of Radix Scutellaria extract in rat plasma using UHPLC with quadrupole time‐of‐flight mass spectrometry and microdialysis intensity‐fading mass spectrometry
Author(s) -
Zhang Xueju,
Liu Shu,
Xing Junpeng,
Pi Zifeng,
Liu Zhiqiang,
Song Fengrui
Publication year - 2018
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700666
Subject(s) - radix (gastropod) , mass spectrometry , chemistry , microdialysis , chromatography , quadrupole time of flight , time of flight mass spectrometry , analytical chemistry (journal) , tandem mass spectrometry , ion , ionization , organic chemistry , biochemistry , botany , extracellular , biology
Radix Scutellaria is a widely used traditional Chinese medicine in the treatment of various diseases. However, the activities of the absorbed components and metabolites of its main flavones in rat plasma need further investigation. In this study, a systematic method based on ultra‐high performance liquid chromatography with quadruple time‐of‐flight mass spectrometry was developed to speculate the absorbed components and metabolites of the main flavonoids in Radix Scutellaria extract in rat plasma sample after oral administration of the extract. Twelve compounds, including four prototype components and eight metabolites, were confirmed in drug‐containing plasma. In these metabolites, five were originally detected in rat plasma. The possible metabolic pathways of these polyhydroxy flavones in vivo were described and clarified. Microdialysis with intensity‐fading mass spectrometry was originally employed to investigate the binding affinities of the absorbed components and metabolites with α‐glucosidase. The order of their binding affinities was P4 > P3 > P2 > P1≥M5 > M3 > M1. The research result is helpful to deepen the understanding of the absorbed components and metabolic pathways of main flavones from Radix Scutellaria, and provide a new approach to screen potential inhibitors from in vivo components originated from Chinese herb.