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Rapid determination of ginkgolic acids in Ginkgo biloba kernels and leaves by direct analysis in real time‐mass spectrometry
Author(s) -
Huang Zhongping,
Xu Yueting,
Huang Yilei,
Liu Charles,
Jiang Kezhi,
Wang Lili
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700626
Subject(s) - ginkgo biloba , chemistry , chromatography , mass spectrometry , detection limit , gas chromatography–mass spectrometry , dart ion source , solvent , time of flight mass spectrometry , ginkgo , analytical chemistry (journal) , ion , electron ionization , organic chemistry , traditional medicine , ionization , medicine , pharmacology
A novel method based on direct analysis in real time integrated with mass spectrometry was established and applied into rapid determination of ginkgolic acids in Ginkgo biloba kernels and leaves. Instrument parameter settings were optimized to obtain the sensitive and accurate determination of ginkgolic acids. At the sample introduction speed of 0.2 mm/s, high intensity of [M–H] – ions for ginkgolic acids were observed in the negative ion mode by utilization of high‐purity helium gas at 450°C. Two microliters of methanol extract of G. biloba kernels or leaves dropped on the surface of Quick‐Strip module was analyzed after solvent evaporated to dryness. A series of standard solutions of ginkgolic acid 13:0 in the range of 2–50 mg/L were analyzed with a correlation coefficient r = 0.9981 and relative standard deviation ( n = 5) from 12.5 to 13.7%. The limit of detection was 0.5 mg/L. The results of direct analysis in real time‐mass spectrometry were in agreement with those observed by thermochemolysis gas chromatography. The proposed method demonstrated significant potential in the application of the high‐throughput screening and rapid analysis for ginkgolic acids in dietary supplements.