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Supercritical fluid chromatography coupled with tandem mass spectrometry: A high‐efficiency detection technique to quantify Taxane drugs in whole‐blood samples
Author(s) -
Jin Chan,
Guan Jibin,
Zhang Dong,
Li Bing,
Liu Hongzhuo,
He Zhonggui
Publication year - 2017
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201700536
Subject(s) - chromatography , chemistry , detection limit , cabazitaxel , supercritical fluid chromatography , mass spectrometry , taxane , repeatability , sample preparation , reproducibility , liquid chromatography–mass spectrometry , extraction (chemistry) , supercritical fluid extraction , tandem mass spectrometry , matrix (chemical analysis) , high performance liquid chromatography , medicine , androgen deprivation therapy , prostate cancer , cancer , breast cancer
Abstract We present a technique to rapid determine taxane in blood samples by supercritical fluid chromatography together with mass spectrometry. The aim of this study was to develop a supercritical fluid chromatography with mass spectrometry method for the analysis of paclitaxel, cabazitaxel, and docetaxel in whole‐blood samples of rats. Liquid‐dry matrix spot extraction was selected in sample preparation procedure. Supercritical fluid chromatography separation of paclitaxel, cabazitaxel, docetaxel, and glyburide (internal standard) was accomplished within 3 min by using the gradient mobile phase consisted of methanol as the compensation solvent and carbon dioxide at a flow rate of 1.0 mL/min. The method was validated regarding specificity, the lower limit of quantification, repeatability, and reproducibility of quantification, extraction recovery, and matrix effects. The lower limit of quantification was found to be 10 ng/mL since it exhibited acceptable precision and accuracy at the corresponding level. All interday accuracies and precisions were within the accepted criteria of ±15% of the nominal value and within ±20% at the lower limit of quantification, implying that the method was reliable and reproducible. In conclusion, this method is a promising tool to support and improve preclinical or clinical pharmacokinetic studies with the taxanes anticancer drugs.